Gaining data on if therapeutic compounds are toxic to cells (cytotoxicity) is really a critical initial step to comprehending the lengthy term viability of some compounds inside a drug discovery program. If your given group of test articles causes cytotoxicity in human or animal cell lines they will not flourish in later animal studies or perhaps in the clinic.
Cytotoxicity screens are utilized at the start of the drug discovery pipeline to recognize lead compounds and eliminate compounds that will probably cause drug failure. Of these early screens there are a variety of assays accustomed to understand potential drug liabilities due cytotoxicity. The very first cytotoxicity assays frequently operated by organizations are classified as “cell viability assays”. Cell viability assays frequently take the type of an inexpensive ATP assay that enables compounds to become quickly be when compared with reference compounds. These early cell viability research is frequently “High throughput” meaning a lot of compounds are go to rank order test articles according to general toxicity.
Additional cytotoxicity screens include assays that study specific mechanisms of toxicity. These mechanistic cytotoxicity screens are frequently pursue initial cell viability studies to provide greater detail on what causes toxicity towards the investigator. However, mechanistic in vitro research comes in a greater cost so usually merely a select quantity of lead compounds are tell you these screens.
Picking out a Contract Research Organization (CRO) to do Cytotoxicity Screens
Complete your early cytotoxicity studies with CRO noted for getting expertise in in vitro toxicology and cell based models.
IONTOX’s cytotoxicity screens vary from predictive multi-endpoint models to plain cell viability screens (ATP) to position order compounds.
Here exist several assays utilized by IONTOX to evaluate cytotoxicity:
Ï Phospholipidosis Steatosis
Ï Oxidative Stress
Ï Cell Viability
Ï Mitotoxicity
Ï Cytotoxicity Panel